Product Description

EPHA5 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67025-50, MB67025-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands. While EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain . Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer . Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimμlate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellμlar domain is sufficient for this function as long as it is clustered . The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells .The EphA3 receptor preferentially binds ephrin-A5. This ligand-receptor interaction stimμlates EphA3 signaling, regμlates cell adhesion and migration, and induces cellμlar morphologic responses . EphA3 plays a critical role in callosal axon guidance , retinotectal mapping of neurons , as well as cardiac cell migration and differentiation . Investigators have shown that somatic mutations in functional domains of EphA3 are linked to lung cancer progression . In addition, EphA3 expression levels have been correlated with tumor angiogenesis and progression in gastric and colorectal carcinoma.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: BSK, EHK1, HEK7, TYRO4, Ephrin type-A receptor 5, Brain-specific kinase, EPH homology kinase 1, EHK-1, EPH-like kinase 7, EK7, hEK7

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human EPHA5. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of EPHA5 protein.

Molecular Weight: ~ 115 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P54756

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only