Product Description

EphB6 (D881) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2450-50, BS2450-100

Sizes: 50µl, 100µl

Swiss-Prot: O15197

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ephrin receptor encoded by this gene lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: EphB6 (D881) polyclonal antibody detects endogenous levels of EphB6 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EphB6 (D881) polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: PC12 whole cell lysate
Lane 4: H9C2 whole cell lkysate, Immunohistochemistry (IHC) analyzes of EphB6 (D881) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing secreted and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 119 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Ephrin type-B receptor 6; HEP; Tyrosine-protein kinase-defective receptor EPH-6; EPHB6

Immunogen: Synthetic peptide, corresponding to amino acids 851-900 of Human EphB6.

Conjugate: Unconjugated

Modification: Unmodified