Product Description

EPO Polyclonal Antibody

Catalogue Numbers: BS60377-50, BS60377-100

Sizes: 50µl, 100µl

Alternative Name: Erythropoietin; INN=Epoetin

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01588

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Erythropoietin, or Epo, is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. In addition, Epo has also been shown to play a role in primitive embryonic erythropoiesis. Epo is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. Circulating Epo is a 165 amino acid glycoprotein. The Epo receptor, EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Overexpression of Epo is associated with several pathophysiological conditions, such as polycythemias vera, which is caused by the Epo-independent growth of erythrocytic progenitors from abnormal stem cells. A deficiency in Epo expression has been associated with afflictions such as anemia of chronic disease (ACD), frequently found in rheumatoid arthritis (RA) patients.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EPO polyclonal antibody detects endogenous levels of EPO protein.

Bioworld Molecular Weight: ~ 21, 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EPO polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human EPO.

Conjugate: Unconjugated

Modification: Unmodified