Eps15 (phospho Tyr849) rabbit pAb - ES5140

Eps15 (phospho Tyr849) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES5140-50, ES5140-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009],

Alternate Name: EPS15; AF1P; Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p

Source: Rabbit

Applications: WB; ELISA

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Reactivity: Human; Mouse; Rat

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Eps15 (phospho Tyr849)

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 100kD

Human Gene ID: 2060

Human SWISS Prot NO: P42566

Subcellular Location: Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes.; [Isoform 2]: Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Colocalizes with HGS on bilayered clathrin coats on endosomes.

Research Use Only