ERα (Phospho-S118) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94082-50, BS94082-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P03372(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Estrogen receptor alpha (ERa, ER, ESR, ESRA, Era, NR3A1, estrogen receptor 1) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. Alternative splicing results in several ERa mRNA transcripts, which differ primarily in their 5' untranslated regions. ERa undergoes phosphorylation in response to estradiol binding. Human ERa is predominately phosphorylated on Ser 118 and to a lesser extent on Ser 104 and Ser 106. In response to activation of the mitogen-activated protein kinase pathway, phosphorylation occurs on Ser 118 and Ser 167. These serine residues are all located within the activation function 1 region of the N-terminal domain of ERa. In contrast, activation of protein kinase A increases the phosphorylation of Ser 236, which is located in the DNA-binding domain. Src kinase-dependent Tyr 537 phosphorylation may enhance estrogen binding to ERa. Mutation of Tyr 537 of the human ERa produces receptors having a range of constitutive activity.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: ERα (Phospho-S118) polyclonal antibody detects endogenous levels of ERα protein only when phosphorylated at S118.
Bioworld Molecular Weight: 66 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Estrogen Receptor alpha(phospho S118) on MCF-7 cell lysates using anti- phospho-Estrogen Receptor alpha(S118) antibody at 1/1,000 dilution., ICC staining phospho-Estrogen Receptor alpha(S118) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: ER, ESR1, DKFZp686N23123, ER alpha, ER, ER-alpha, Era, ESR, ESR1, ESR1, ESRA, Estradiol receptor, Estrogen nuclear receptor alpha, Estrogen receptor 1, Estrogen receptor alpha 3*, 4, 5, 6, 7*/822 isoform, Estrogen receptor alpha, Estrogen receptor alpha delta 3*, 4, 5, 6, 7*, 8*/941 isoform, Estrogen receptor alpha delta 3*, 4, 5, 6, 7*/819 2 isoform, Estrogen receptor alpha delta 4 +49 isoform, Estrogen receptor alpha delta 4*, 5, 6, 7*/654 isoform, Estrogen receptor alpha delta 4*, 5, 6, 7, 8*/901 isoform, Estrogen receptor alpha E1 E2 1 2, Estrogen receptor alpha E1 N2 E2 1 2, Estrogen receptor, ESTRR, NR3A1, Nuclear receptor subfamily 3 group A member 1,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser118 of human Estrogen Receptor alpha.
Conjugate: Unconjugated
Modification: Phosphorylation