Product Description

ERAB (E135) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1713-50, BS1713-100

Sizes: 50µl, 100µl

Swiss-Prot: Q99714

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: β-Amyloid is a neurotoxic peptide that is associated with the pathogenesis of Alzheimer’s disease. β-Amyloid aggregates induce cell death of neurons through the disruption of cell membranes and the generation of reactive oxygen intermediates.These neurotoxic effects are also attributed to the interaction of β-Amyloid with intracellular proteins, specifically ERAB, the endoplasmic reticulum-associated β-Amyloid-binding protein. ERAB is characterized as a NAD+-dependent dehydrogenase that is constitutively expressed in tissues and overexpressed in neurons affected in Alzheimer’s disease. Cells overexpressing ERAB in vitro have been shown to be more sensitive to β-Amyloid-induced stress, and blocking the activity of ERAB has been shown to inhibit this cell death, indicating that β-Amyloid induced cell death is mediated by ERAB.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ERAB (E135) polyclonal antibody detects endogenous levels of ERAB protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ERAB (E135) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate, Immunohistochemistry (IHC) analyzes of ERAB (E135) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 27 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: 3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; Endoplasmic reticulum-associated amyloid beta-peptide-binding protein; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Short-chain type dehydrogenase/reductase XH98G2; Type II HADH; HSD17B10; ERAB; HADH2; MRPP2; SCHAD; XH98G2

Immunogen: Synthetic peptide, corresponding to amino acids 102-150 of Human ERAB.

Conjugate: Unconjugated

Modification: Unmodified