Product Description

ErbB2/HER2 (phospho-Y1221/Y1222) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4089-50, BS4089-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Neu, a glycoprotein, undergoes transactivation upon hetero-dimerization with other EGF receptor family members. Neu heterodimerization with ErbB-3 recruits heregμlin, which induces phosphoinositide (PI) 3-kinase activation. Activation of Neu potentiates tumor cell motility and protease secretion and invasion, and also modμlates cell cycle checkpoint function, DNA repair and apoptotic responses.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD340, ERBB2, HER2, MLN19, NEU, NGL

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human ErbB2/HER2 around the phosphorylation site of Tyrosine 1221/1222.

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-ErbB2/HER2 (Y1221/Y1222) polyclonal antibody detects endogenous levels of ErbB2/HER2 protein only when phosphorylated at Tyr1221 and Tyr1222.

Molecular Weight: ~ 138, 185 kDa

Pathway: ErbB HER Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P04626

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only