ERCC1 Monoclonal Antibody
Catalogue Numbers: MB66595-50, MB66595-100
Sizes: 50µl, 100µl
Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Swiss-Prot: P07992
Host: Mouse
Reactivity: Human
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals. Excision Repair Cross Complementing 1 (ERCC1) forms a complex with ERCC4/XPF, which acts as the 5’ endonuclease required to excise the lesion. ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) and involved in repair of double strand breaks. Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC).
Purification and Purity: The antibody was purified by immunogen affinity chromatography.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of ERCC1 protein.
Extra Notes: Western blot analysis of ERCC1 expression in T47D (A), ZR751 (B), CaCO2 (C), Molt4 (D) whole cell lysates.
Bioworld Molecular Weight: ~ 41 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: DNA excision repair protein ERCC-1
Immunogen: Purified recombinant human ERCC1 protein fragments expressed in E.coli.
Conjugate: Unconjugated
Modification: Unmodified