ERCC1 Monoclonal Antibody-MB66832

ERCC1 Monoclonal Antibody

Catalogue Numbers: MB66832-50, MB66832-100

Sizes: 50µl, 100µl

Product: Mouse IgM kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P07992

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer; it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5' incisions during DNA repair. The ERCC1/XPF endonuclease preferentially cleaves one strand of DNA between duplex and single-stranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of ERCC1 protein.

Extra Notes: Western blot analysis of ERCC1 expression in A549 (A), Hela (B), T47D (C) whole cell lysates.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: DNA excision repair protein ERCC-1

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ERCC1. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified