ERCC1 Polyclonal Antibody-BS1111

ERCC1 Polyclonal Antibody

Catalogue Numbers: BS1111-50, BS1111-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P07992

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals. Excision Repair Cross Complementing 1 (ERCC1) forms a complex with ERCC4/XPF, which acts as the 5’ endonuclease required to excise the lesion. ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) and involved in repair of double strand breaks. Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ERCC1 polyclonal antibody detects endogenous levels of ERCC1 protein.

Extra Notes: Western blot (WB) analysis of ERCC1 polyclonal antibody at 1:500 dilution
Lane1:U-87MG whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:C6 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: COFS4; RAD10; UV20

Immunogen: Synthetic peptide, corresponding to Human ERCC1.

Conjugate: Unconjugated

Modification: Unmodified