Product Description

ERCC1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8468-50, BS8468-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07992

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB 1:500 - 1:2000
IF 1:50 - 1:200

Background: Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer; it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5' incisions during DNA repair. The ERCC1-XPF endonuclease preferentially cleaves one strand of DNA between duplex and single-stranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ERCC1 polyclonal antibody detects endogenous levels of ERCC1 protein.

Bioworld Molecular Weight: ~ 38kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using ERCC1 antibody., Immunofluorescence analysis of HeLa cells using ERCC1 antibody., Immunofluorescence analysis of U2OS cells using ERCC1 antibody.Blue: DAPI for nuclear staining.

Alternative Name: DNA excision repair protein ERCC-1; : ERCC1

Immunogen: Recombinant full length Human ERCC1.

Conjugate: Unconjugated

Modification: Unmodified