ERCC4 (A822) Polyclonal Antibody-BS2430

ERCC4 (A822) Polyclonal Antibody

Catalogue Numbers: BS2430-50, BS2430-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92889

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Xeroderma Pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer, and it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, is a 115 kDa protein that associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC-1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5’ incisions during DNA repair. The ERCC1-XPF endonuclease preferentially cleaves one strand of DNA between duplex and singlestranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: XPF (A822) polyclonal antibody detects endogenous levels of XPF protein.

Extra Notes: Western blot (WB) analysis of ERCC4 (A822) pAb at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:U-87MG whole cell lysate(40ug)
Lane4:C6 whole cell lysate(40ug)
Lane5:CT26 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 117 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: ERCC4; ERCC11; XPF; DNA repair endonuclease XPF; DNA excision repair protein ERCC-4

Immunogen: Synthetic peptide, corresponding to amino acids 796-840 of Human ERCC4.

Conjugate: Unconjugated

Modification: Unmodified