ERCC5/XPG (E160) Polyclonal Antibody-BS1973

ERCC5/XPG (E160) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1973-50, BS1973-100

Sizes: 50µl, 100µl

Swiss-Prot: P28715

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: Seven complementation groups (A-G) of xeroderma pigmentosum have been described. The xeroderma pigmentosum group A protein, XPA, is a zinc metalloprotein which preferentially binds to DNA damaged by ultraviolet (UV) radiation and chemical carcinogens. XPA is a DNA repair enzyme that has been shown to be required for the incision step of nucleotide excision repair. XPG (also designated ERCC5) is an endonuclease that makes the 3’ incision in DNA nucleotide excision repair. Mammalian XPG is similar in sequence to yeast RAD2. Conserved residues in the catalytic center of XPG are important for nuclease activity and function in nucleotide excision repair.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: XPG (E160) polyclonal antibody detects endogenous levels of XPG protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ERCC5/XPG (E160) pAb at 1:1000 dilution
Lane 1: PC3 whole cell lysate (40ug)
Lane 2: SGC7901 whole cell lysate (20ug)
Lane 3: HCT116 whole cell lysate (20ug), Immunohistochemistry (IHC) analyzes of ERCC5/XPG (E160) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 133, 200 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: COFS 3; COFS3; DNA excision repair protein ERCC 5; DNA excision repair protein ERCC-5; ERCC 5; ERCC5; ERCC5; ERCM 2; ERCM2; Excision Repair Cross Complementing Rodent Repair Deficiency; XPG; XPG complementing protein; XPGC;

Immunogen: Synthetic peptide, corresponding to amino acids 130-180 of Human ERCC5/XPG.

Conjugate: Unconjugated

Modification: Unmodified