Product Description

ERI1 (D298) Polyclonal Antibody

Catalogue Numbers: BS9202-50, BS9202-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8IV48

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Helicase with RNase motif, more commonly designated Dicer, cleaves doublestranded RNA (dsRNA) in the RNA interference and small temporal RNA (stRNA) pathways, producing active small RNA components (siRNAs) which target the destruction of RNA and repress gene expression. Human Dicer cleaves dsRNA independent of ATP. The 3’-5’ exonuclease ERI-1, also known as Protein 3’hExo, degrades Histone mRNA after replication and may be involved in the regulation of RNA interference. ERI-1 has a high affinity for the stem-loop structure of replication-dependent Histone pre-mRNAs. It requires the 5’-ACCCA-3’ sequence present in stem-loop structure. ERI-1 and a stemloop binding protein (SLBP) target opposite faces of a unique highly conserved stem-loop RNA scaffold towards the 3’ end of Histone mRNA.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ERI1 (D298) polyclonal antibody detects endogenous levels of ERI1 protein.

Extra Notes: Western blot (WB) analysis of ERI1 pAb at 1:500 dilution
Lane1:SGC7901 whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:U-87MG whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: 3'EXO; THEX1; HEXO; Eri-1 homolog; 3'-5' exoribonuclease 1;

Immunogen: Synthetic peptide, corresponding to amino acids 266-318 of Human ERI1.

Conjugate: Unconjugated

Modification: Unmodified