Product Description

ERK 8 (phospho Thr175/Y177) Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA21092-50, RA21092-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein., domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation., domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases., enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1., function:In vitro, phosphorylates MBP., PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro., PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase., similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily., similarity:Contains 1 protein kinase domain., subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1., tissue specificity:Widely expressed with a maximal expression in lung and kidney.,

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC-p, IF (paraffin section), ELISA

Alternate Names: MAPK15, ERK7, ERK8, Mitogen-activated protein kinase 15, MAP kinase 15, MAPK 15, Extracellular signal-regulated kinase 7, ERK-7, Extracellular signal-regulated kinase 8, ERK-8

Clonality: Polyclonal

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight: 60

Protein Molecular Weight (kDa): 60

Storage: -20°C/1 year

Human Gene ID: 225689

Human SwissProt Number: Q8TD08

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only