ERK 8 (phospho Thr175/Y177) rabbit pAb - ES5241

ERK 8 (phospho Thr175/Y177) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES5241-50, ES5241-100

Citations, Manuals and MSDS Available upon request.

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein., domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation., domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases., enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1., function:In vitro, phosphorylates MBP., PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro., PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase., similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily., similarity:Contains 1 protein kinase domain., subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1., tissue specificity:Widely expressed with a maximal expression in lung and kidney.,

Alternate Name: MAPK15; ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8

Source: Rabbit

Applications: WB; IHC; IF; ELISA

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Reactivity: Human; Mouse

Immunogen: The antiserum was produced against synthesized peptide derived from human ERK8 around the phosphorylation site of Thr175 and Tyr177. AA range:141-190

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 60kD

Human Gene ID: 225689

Human SWISS Prot NO: Q8TD08

Subcellular Location: Cytoplasm, cytoskeleton, cilium basal body. Cell junction, tight junction. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasmic vesicle, autophagosome. Golgi apparatus. Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936). Translocates to the nucleus upon activation (PubMed:20638370). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity).

Research Use Only