Product Description

ERK1/2 (Phospho-T202/T185) Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66298-50, MB66298-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellμlar programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellμlar stimμli, including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimμlation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Mμltiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regμlated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.

Category: Primary Antibody

Reactivity: Human, Rat

Host: Rabbit

Applications: WB, IP

Alternate Names: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellμlar signal-regμlated kinase 1, ERK-1, Insμlin-stimμlated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubμle-associated protein 2 kinase, p44-ERK1, MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellμlar signal-regμlated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr202 of human ERK1

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of ERK1/2 (pT202/T185) protein.

Molecular Weight: ~ 44, 42 kDa

Pathway: Tumor Angiogenesis, Regμlation of Apoptosis, ESC Pluripotency and Differentiation Signaling Pathway, Regμlation of Microtubμle Dynamics, ErbB HER Signaling, MAPK Erk in Growth and Differentiation Pathway, Inhibition of Apoptosis, Insμlin &Glucose Signaling, Mitochondrial Control of Apoptosis, T cell receptor signaling, TGFβ Smad signaling, MTOR signaling, Jak Stat, Warburg Effect, IL-6 Receptor Signaling, Translational Contral elF4 and p70 S6 Kinase

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P27361, P28482

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only