Product Description

ERK1/2 (R172) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2265-50, BS2265-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellμlar-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase (MEK), MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regμlation and development.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellμlar signal-regμlated kinase 1, ERK-1, Insμlin-stimμlated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubμle-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellμlar signal-regμlated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 140-190 of Human ERK 2.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ERK 2 (R172) polyclonal antibody detects endogenous levels of ERK 2 protein. This antibody also recognizes ERK1 protein.

Molecular Weight: ~ 42 kDa

Pathway: Immune Cell Signaling, Warburg Effect, Regμlation of Microtubμle Dynamics, T cell receptor signaling, Inhibition of Apoptosis, Insμlin &Glucose Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway, Mitochondrial Control of Apoptosis, ErbB HER Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P28482

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only