Product Description

ERK1/2 (R189) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1968-50, BS1968-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellμlar-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regμlated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for fμll enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regμlators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellμlar signal-regμlated kinase 1, ERK-1, Insμlin-stimμlated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubμle-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellμlar signal-regμlated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human ERK1.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ERK1/2 (R189) polyclonal antibody detects endogenous levels of ERK1/2 protein.

Molecular Weight: ~ 42, 44 kDa

Pathway: Immune Cell Signaling, Warburg Effect, Regμlation of Microtubμle Dynamics, T cell receptor signaling, Inhibition of Apoptosis, Insμlin &Glucose Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway, Mitochondrial Control of Apoptosis, ErbB HER Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P27361/P28482

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only