ERK5 Polyclonal Antibody-BS90475

ERK5 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90475-50, BS90475-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13164(Human) Q9WVS8(Mouse) P0C865(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
FC:1:50-1:100

Background: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ERK5 polyclonal antibody detects endogenous levels of ERK5 protein.

Bioworld Molecular Weight: 115 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ERK5 on Hela cells lysates using anti-ERK5 antibody at 1/1,000 dilution., ICC staining ERK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MAP kinase 7, MAPK 7, BMK-1, ERK-5, MAPK7, Big MAP kinase 1, BMK 1, BMK 1 kinase, BMK-1, BMK1, BMK1 Kinase, EC 2.7.11.24, ERK 4, ERK 5, ERK-5, ERK4, ERK5, Extracellular signal regulated kinase 5, Extracellular signal-regulated kinase 5, MAP kinase 7, MAPK 7, MAPK7, Mitogen activated protein kinase 7, Mitogen-activated protein kinase 7, MK07, OTTHUMP00000065906, OTTHUMP00000065907, PRKM 7, PRKM7, PROTEIN KINASE, MITOGEN-ACTIVATED, 7,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified