Estrogen Receptor alpha Monoclonal Antibody-MB10892

Estrogen Receptor alpha Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10892-50, MB10892-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P03372

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-F, IHC-P, ICC/IF, IP, , ChIP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20

Background: ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 66 kDa; Observed MW: 66 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Estrogen Receptor alpha in K562, Hela lysates using Estrogen Receptor alpha antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Estrogen Receptor alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunocytochemistry analysis of Estrogen Receptor alpha in MCF-7 using Estrogen Receptor alpha antibody, and DAPI

Alternative Name: ESR1; Era; Eralpha; Estrogen receptor; Estradiol receptor; ER-alpha; Estrogen receptor 1; NR3A1; ER; ESR; ESRA; Estrogen receptor alpha

Immunogen: A synthetic peptide of human Estrogen Receptor alpha

Conjugate: Unconjugated

Modification: Unmodified