Product Description

Estrogen Receptor alpha Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66340-50, MB66340-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains. Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regμlates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation at mμltiple sites provides an important mechanism to regμlate ERα activity. Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regμlating ERα activity. Ser118 may be the substrate of the transcription regμlatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt. According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC, IF/ICC, IP, ChIP

Alternate Names: ESR, NR3A1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide of human Estrogen Receptor alpha

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50), ChIP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Estrogen Receptor alpha protein.

Molecular Weight: ~ 65 kDa

Pathway: MAPK Erk in Growth and Differentiation Pathway

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P03372

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only