Estrogen Receptor beta (Phospho-S105) Polyclonal Antibody-BS64525

Estrogen Receptor beta (Phospho-S105) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64525-50, BS64525-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92731

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Background: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Estrogen Receptor beta (Phospho-S105) polyclonal antibody detects endogenous levels of Estrogen Receptor beta protein only when phosphorylated at Ser105.

Bioworld Molecular Weight: ~ 59 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Estrogen Receptor beta (Phospho-S105) polyclonal antibody at 1:500 dilution
Lane A: HEK293T whole cell lysate
Lane B: Hela whole cell lysate
Lane C: A375 whole cell lysate
Lane D: The Spleen tissue lysate of Mouse
Lane E: The Spleen tissue lysate of Rat
Lane F: The Testis tissue lysate of Rat

Alternative Name: Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2; ESR2; ESTRB; NR3A2; ER-β

Immunogen: Synthetic phosphopeptide derived from human Estrogen Receptor beta around the phosphorylation site of Serine 105.

Conjugate: Unconjugated

Modification: Phosphorylation