Product Description

Et V4 Polyclonal Antibody

Catalogue Numbers: BS61024-50, BS61024-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P43268

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Several members of the Ets gene family are known to encode sequence-specific DNA binding proteins. These include mouse PU.1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-a. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU.1, although PU.1 binds equally well to both sequences. It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of which is expressed primarily in cells of hematopoietic origin.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Et V4 polyclonal antibody detects endogenous levels of Et V4 protein.

Extra Notes: Western blot (WB) analysis of Et V4 pAb at 1:500 dilution
Lane1:H9C2 whole cell lysate(40ug)
Lane2:CT-26 whole cell lysate(40ug)
Lane3:Hela whole cell lysate(40ug)
Lane4:A549 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 59 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: ETS translocation variant 4; Adenovirus E1A enhancer-binding protein; E1A-F; Polyomavirus enhancer activator 3 homolog; Protein PEA3; Et V4; E1AF; PEA3

Immunogen: A synthetic peptide corresponding to residues in Human Et V4.

Conjugate: Unconjugated

Modification: Unmodified