ETAR (N427) Polyclonal Antibody-BS2597

ETAR (N427) Polyclonal Antibody

Catalogue Numbers: BS2597-50, BS2597-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P25101

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Endothelin receptor A (ETAR), also known as EDNRA, ET1 receptor, ETA, EDN1 and ET-AR, is a member of the guanine-binding regulatory protein-coupled receptor family. ETAR binds endothelins and has the highest affinity for its ligand, ET1, as compared to the ETBR receptor. Both ET receptors, ETAR and ETBR, are activated by ET1, which results in inhibition of active lens sodiumpotassium transport. Activation of the ET receptors also causes an increase in cytoplasmic calcium concentration in cultured lens epithelial cells. In addition,ETAR induces arachidonic acid accumulation. ETAR has seven hydrophobic transmembrane domains and is expressed in aorta, lung, atrium, kidney, placenta and prostate. Specifically, placental vascular smooth muscle cells (PVSMCs) exclusively express ETAR.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ETAR (N427) polyclonal antibody detects endogenous levels of ETAR protein.

Extra Notes: Western blot (WB) analysis of ETAR (N427) pAb at 1:500 dilution
Lane1:C6 whole cell lysate(40ug)
Lane2:BV2 whole cell lysate(40ug)
Lane3:HCT116 whole cell lysate(40ug)
Lane4:SGC7901 whole cell lysate(40ug)
Lane5:A549 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Endothelin-1 receptor; Endothelin A receptor; ET-A; ETA-R; hET-AR; EDNRA; ETA; ETRA

Immunogen: Synthetic peptide, corresponding to amino acids 402-452 of Human ETAR.

Conjugate: Unconjugated

Modification: Unmodified