Product Description

ETK (L562) Polyclonal Antibody

Catalogue Numbers: BS1537-50, BS1537-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51813

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: ETK, also known as BMX, is a member of the Bruton's tyrosine kinase (Btk) family. It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways. Phosphorylation of Tyr566 by Src kinase is required for activation of Etk in vivo. In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at Tyr40.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ETK (L562) polyclonal antibody detects endogenous levels of ETK protein.

Extra Notes: Western blot (WB) analysis of BMX pAb at 1:500 dilution
Lane1:Hela whole cell lysate(40ug)
Lane2:SHSY5Y whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 78 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Cytoplasmic tyrosine-protein kinase BMX; Bone marrow tyrosine kinase gene in chromosome X protein; Epithelial and endothelial tyrosine kinase; NTK38; BMX

Immunogen: Synthetic peptide, corresponding to amino acids 550-600 of Human ETK.

Conjugate: Unconjugated

Modification: Unmodified