Product Description

EV71 3D Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78092-50, BS78092-100

Host: Rabbit

Reactivity: Other (Wide Range)

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Enterovirus A 71 (EV71) is a neurotropic virus that may lead to acute flaccid paralysis, encephalitis, cardiopulmonary failure or even death. No vaccine and defensive drug controlling EV71 is currently available, novel and efficient antiviral drug or vaccine is therefore urgently needed. 3Dpol (RNA-dependent RNA polymerase (RdRp)) has been an important target for anti-EV71 drug development.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of HeLa cells, using EV71 3D antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Bioworld Molecular Weight: 51KDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: Recombinant protein of Enterovirus EV713D.

Conjugate: Unconjugated

Modification: Unmodified