Product Description

EWSR1 Polyclonal Antibody

Catalogue Numbers: BS5715-50, BS5715-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q01844

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: EWS is a nuclear RNA-binding protein. As a result of chromosome translocation, the EWS gene is fused to a variety of transcription factors, including ATF-1 in human neoplasias. In the Ewing family of tumors, the N-terminal domain of EWS is fused to the DNA-binding domain of various ETS transcription factors, including Fli-1, Erg, Et V1, E1AF and FEV. The EWS/Fli-1 chimeric protein acts as a more potent transcriptional activator than Fli-1 and can promote cell transformation. Two functional regions have been identified in EWS; an amino-terminal region (domain A), that has little transactivation activity but transforms efficiently when fused to Fli-1, and a distal region (domain B), which shows transactivation activity but transforms less efficiently when fused to Fli-1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EWSR1 polyclonal antibody detects endogenous levels of EWSR1 protein.

Extra Notes: Western blot (WB) analysis of EWSR1 pAb at 1:2000 dilution
Lane1:SGC7901 whole cell lysate(40ug)
Lane2:PC3 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 95 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: RNA-binding protein EWS; EWS oncogene; Ewing sarcoma breakpoint region 1 protein; EWS

Immunogen: Synthetic peptide, corresponding to the 465-510 of Human EWSR1.

Conjugate: Unconjugated

Modification: Unmodified