Product Description

Exo1 (K86) Polyclonal Antibody

Catalogue Numbers: BS2536-50, BS2536-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UQ84

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1 (Exonuclease I) proteins are important contributors to chromosome processing during mammalian DNA repair and recombination. In mice, the mExo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous human HEX1/ hEXO1 gene to chromosome 1q42-q43. mExo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. In both mammalian and yeast systems, Exo1 is a 5'-3' double stranded DNA exonuclease that has previously been implicated in DNA mismatch repair (MMR). The mismatch repair (MMR) system ensures genome integrity by removing mispaired and unpaired bases that originate during replication. In humans, Exo1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease in MM. In both mammalian and yeast systems, Exo1 plays a structural role in MMR and stabilizes multiprotein complexes containing a number of MMR proteins.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Exo1 (K86) polyclonal antibody detects endogenous levels of Exo1 rotein.

Extra Notes: Western blot (WB) analysis of Exo1 (K86) pAb at 1:500 dilution
Lane1:The Testis tissue lysate of Rat(40ug)
Lane2:The Testis tissue lysate of Mouse(40ug)
Lane3:K562 whole cell lysate(10ug)
Lane4:HEK293T whole cell lysate(10ug)

Bioworld Molecular Weight: ~ 94 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Exonuclease 1; hExo1; Exonuclease I; hExoI; EXO1; EXOI; HEX1

Immunogen: Synthetic peptide, corresponding to amino acids 55-05 of Human Exo1.

Conjugate: Unconjugated

Modification: Unmodified