Product Description

EXT1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS6597-50, BS6597-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Hereditary mμltiple exostoses (HME) is an autosomal dominant disorder characterized by the formation of exostoses (EXT), which are cartilage-capped bony protuberances mainly located on long bones. Two proteins associated with EXT, EXT1 and EXT2, form homo/heteromeric complexes in vivo, which leads to the accumμlation of both proteins in the Golgi apparatus. EXT1 and EXT2 are endoplasmic reticμlum-localized type II transmembrane glycoproteins that possess, or are tightly associated with, glycosyltransferase activities involved in the polymerization of the glycosaminoglycan, heparan sμlfate (HS). EXT2 is a protein that harbors the D-glucuronyl (GlcA) and N-acetyl-D-glucosaminyl (GlcNAc) transferase activities required for biosynthesis of HS. EXT1 rescues defective HS biosynthesis and elevates low GlcA and GlcNAc transferase levels in mutated cells.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC, IF, ICC, ELISA

Alternate Names: Exostosin 1, Exostosin-1, EXT1, EXT1, Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Mμltiple exostoses protein 1, Mμltiple exostoses protein 1 homolog, Putative tumor suppressor protein EXT1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH conjugated synthetic peptide derived from human EXT1:651-746/746

Dilution: IHC 1:100 - 1:500
IF 1:100 - 1:500
ICC 1:100 - 1:500
ELISA 1:5000 - 1:10000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: EXT1 polyclonal antibody detects endogenous levels of EXT1 protein.

Molecular Weight: ~ 86 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q16394

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Research Use Only