EYFP rabbit pAb - ES20756

EYFP rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES20756-50, ES20756-100

Citations, Manuals and MSDS Available upon request.

Background: Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.

Alternate Name: YM3229

Source: Rabbit

Applications: WB

Dilution: WB: 1:3000-5000

Reactivity: Species independent

Immunogen: Recombinant Protein of EYFP

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Research Use Only