Product Description

Ezrin (phospho-T567)/Radixin (phospho-T564)/Moesin (phospho-T558) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4717-50, BS4717-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Ezrin, Moesin and Radixin belong to a family of highly homologous Actinassociated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: Ezrin, Cytovillin, Villin-2, Villin2, Villin 2, p81, EZR, VIL2, Moesin, Membrane-organizing extension spike protein, MSN, Radixin, RDX

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic phosphopeptide derived from human Ezrin, Radixin, Moesin respectively are around the phosphorylation site of Threonine 567, 564, 558.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Ezrin(T567)/Radixin (T564)/Moesin (T558) polyclonal antibody detects endogenous levels of Ezrin, Radixin and Moesin only when phosphorylated at Thr567, Thr564 or Thr558, respectively.

Molecular Weight: ~ 70 kDa

Pathway: Contribution of ECM and Cytoskeletal Factors to EMT, B cell receptor signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P15311/P26038/P35241

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only