F5 Polyclonal Antibody-BS60797

F5 Polyclonal Antibody

Catalogue Numbers: BS60797-50, BS60797-100

Sizes: 50µl, 100µl

Alternative Name: Coagulation factor V; Activated protein C cofactor; Proaccelerin, labile factor; F5

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P12259

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and factors X, IX, V, and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation factor V (Factor V, FV, proaccelerin, labile factor) is a 2,196 amino acid, single chain glycoprotein that is cleaved by thrombin to yield an active, Ca2+ dependent dimer. This heterodimer is essential to the blood coagulation cascade. Together with catalytic Factor Xa and Ca2+ on the surface of platelets or endothelial cells, Factor Va coordinates in a prothrombinase complex, which mediates proteolysis of prothrombin into active thrombin. Due to both the procoagulant properties of Factor V in coordinating proteolytic activation of thrombin, and anticoagulant properties as a cofactor to activated protein C (APC), which selectively destroys FVa and FXa, alterations at the Factor V locus can contribute to hemorrhagic diathesis or thrombosis, respectively.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: F5 polyclonal antibody detects endogenous levels of F5 protein.

Bioworld Molecular Weight: ~ 252 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of F5 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human F5.

Conjugate: Unconjugated

Modification: Unmodified