F8 (S2194) Polyclonal Antibody-BS1119

F8 (S2194) Polyclonal Antibody

Catalogue Numbers: BS1119-50, BS1119-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P00451

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and Factors X, IX, V, and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation Factor VII (serum prothrombin conversion accelerator, proconvertin, F7, Factor VII) is a 406 amino acid, vitamin K-dependent, single chain serine protease that is synthesized in the liver and circulates as an inactive precursor. Factor IX A, Factor X A, Factor XII A, or thrombin mediated proteolytic cleavage of Factor VII at Arg152-Ile153 generates Factor VII A, an active serine protease composed of a catalytic heavy chain disulfide linked to a light chain, containing 2 EGF-like domains. Mutations at the F7 locus that lead to Factor VII deficiencies are generally asymptomatic or phenotypically uncharacterized, with hemorrhagic diathesis occurring at extremely low levels.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: F8 (S2194) polyclonal antibody detects endogenous levels of Factor VIII protein.

Extra Notes: Western blot (WB) analysis of F8 (S2194) pAb at 1:500 dilution
Lane1:PC12 whole cell lysate(40ug)
Lane2:CT26 whole cell lysate(40ug)
Lane3:Hela whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 267 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant component; F8C; Factor VIII; FactorVIII; Factor8; Factor 8; Factor-VIII; Factor-8

Immunogen: Synthetic peptide, corresponding to amino acids 2161-2210 of Human F8.

Conjugate: Unconjugated

Modification: Unmodified