Factor XIIIa Monoclonal Antibody-MB66006

Factor XIIIa Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66006-50, MB66006-100

Product: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: P00488

Host: Mouse

Reactivity: Human

Applications: IHC

All Applications: IHC (1/100 - 1/300)

Background: Factor XIIIA, or fibrin stabilizing factor, is activated by thrombin and Ca2+ to a protransglutaminase in the final step of the blood coagulation cascade that cross-links fibrin chains. This enzyme catalyzes the gamma-glutamyl-epsilon-lysine cross-linking between fibrin molecules, stabilizing the fibrin clot. It is widely expressed in dermal dendritic cells. High expression of Factor XIIIA positive cells can be indicative of inflammatory conditions such as lichen planus and psoriasis.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Factor XIIIa protein.

Bioworld Molecular Weight: ~ 83 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of Factor XIIIa staining in human malignant fibrous histiocytoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of Factor XIIIa staining in human appendix formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of Factor XIIIa staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Alternative Name: F13A; Coagulation factor XIII A chain; Coagulation factor XIIIa; Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Factor XIIIa. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified