Product Description

FAK (Phospho-Y397) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94066-50, BS94066-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q05397(Human) P34152(Mouse) O35346(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF

All Applications: WB:1:1,000
ICC:1:50-1:200

Background: Activation of integrins in the extracellular matrix (ECM) of eukaryotic cells promotes the formation of membrane adhesion complexes, known as focal adhesions, which can include cytoskeletal proteins and protein tyrosine kinases, such as focal adhesion kinase (FAK). Phosphorylation events occurring within focal adhesions influence numerous processes that include mitogenic signaling, cell survival, and cell motility. FAK is a non-receptor tyrosine kinase that is ubiquitously expressed and highly conserved between species. FAK is recruited by Integrin clusters and variably phosphorylated depending on the effector molecules present in the focal adhesion. Phosphorylation of FAK Tyr 397 decreases during serum starvation, contact inhibition, and cell cycle arrest, all conditions under which activating FAK Tyr 407 phosphorylation increases.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: FAK (Phospho-Y397) polyclonal antibody detects endogenous levels of FAK protein only when phosphorylated at Y397.

Bioworld Molecular Weight: 119 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining phospho-FAK (Y397) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining phospho-FAK (Y397) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: FADK 1, FRNK, PPP1R71, PTK2, FADK 1, FADK, FAK related non kinase polypeptide, FAK1, FAK1, Focal adhesion kinase 1, Focal adhesion Kinase, Focal adhesion kinase isoform FAK Del33, Focal adhesion kinase related nonkinase, FRNK, p125FAK, pp125FAK, PPP1R71, Protein phosphatase 1 regulatory subunit 71, Protein tyrosine kinase 2, Protein-tyrosine kinase 2, Ptk2, PTK2 protein tyrosine kinase 2,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr397 of human FAK.

Conjugate: Unconjugated

Modification: Phosphorylation