FAK Rabbit Monoclonal Antibody-BS9850M

FAK Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9850M-50, BS9850M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q05397

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Background: Focal adhesion kinase was initially identified as a major 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of FAK and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 125 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FAK Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: The spleen tissue lysate of Mouse, Immunohistochemical analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin., ICC staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS

Alternative Name: Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK; PTK2; FAK; FAK1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified