Product Description

FAM65B Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71804-50, BS71804-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y4F9

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes an atypical inhibitor of the small G protein RhoA. Inhibition of RhoA activity by the encoded protein mediates myoblast fusion and polarization of T cells and neutrophils. The encoded protein is a component of hair cell stereocilia that is essential for hearing. A splice site mutation in this gene results in hearing loss in human patients.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 118kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using FAM65B antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of Mouse spleen using FAM65B antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of Rat spleen using FAM65B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: RIPOR2; C6orf32; DFNB104; DIFF40; DIFF48; FAM65B; MYONAP; PL48

Immunogen: Recombinant fusion protein of human FAM65B(NP_056948.2).

Conjugate: Unconjugated

Modification: Unmodified