FANCA (phospho-S1149) Polyclonal Antibody-BS4685

FANCA (phospho-S1149) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4685-50, BS4685-100

Swiss-Prot: O15360

Host: Rabbit

FANCA (phospho-S1149) Polyclonal Antibody-BS4685

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200
IP: 1:50~1:200

Background: Fanconi anemia (FA) is an autosomal recessive disorder characterized by bone marrow failure, birth defects and chromosomal instability. At the cellular level, FA is characterized by spontaneous chromosomal breakage and a unique hypersensitivity to DNA cross-linking agents. At least eight complementation groups (A-G) have been identified and six FA genes (for subtypes A, C, D2, E, F and G) have been cloned. The FA proteins lack sequence homologies or motifs that could point to a molecular function. The cellular accumulation of FA proteins, including FANCA and FANCG, is subject to regulation by TNFα signaling. Phosphorylation of FANC (Fanconi anemia complementation group) proteins is thought to be important for the function of the FA pathway. FANCA, also known as FACA and FANCH, associates with the Brm-related gene 1 (BRG1) product, a subunit of the SWI/SNF complex which remodels chromatin structure through a DNA-dependent ATPase activity. FANCA is mainly expressed in lymphoid tissues, testis and ovary. The amino-terminal region of the FANCA protein is required for FANCG binding, FANCC binding, nuclear localization and functional activity of the complex.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-FANCA (S1149) polyclonal antibody detects endogenous levels of FANCA protein only when phosphorylated at Ser1149.

Bioworld Molecular Weight: ~ 163 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-FANCA (S1149) polyclonal antibody at 1:500 dilution
Lane 1: HepG2 cell lysate treated with EGF (0.1ng/ML,30mins)
Lane 2: sp2/0 cell lysate treated with EGF (0.1ng/ML,30mins)
Lane 3: H9C2 cell lysate treated with EGF (0.1ng/ML,30mins), Immunohistochemistry (IHC) analyzes of p-FANCA (S1149) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Fanconi anemia group A protein; Protein FACA; FAA; FACA; FANCH

Immunogen: Synthetic phosphopeptide derived from human FANCA around the phosphorylation site of Serine 1149.

Modification: Phosphorylation

Conjugate: Unconjugated