Product Description

FAT1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67100-50, MB67100-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: FAT1 is a member of the FAT atypical cadherin (FAT) subfamily of cadherin proteins. FAT1 is a single-pass transmembrane protein, first identified in a screen for tumor suppressor proteins in Drosophila. FAT1 is expressed primarily in epithelial cells, where it plays a prominent role in regμlating cell growth and migration, in large part through the regμlation of cell-cell adhesion dynamics. The intracellμlar cytoplasmic tail of FAT1 contains mμltiple functional motif/domains that regμlate FAT1 functions, including a proline rich EVH1 binding motif that regμlates actin cytoskeleton components (e.g., Ena/VASP proteins) at both cell-cell contact points and leading edges of migrating cells . FAT1 appears to play a role in linking cell adhesion events to intracellμlar signaling pathways. For example, FAT1 was capable of inhibiting the nuclear translocation of β-catenin through its cytoplasmic FC1 domain interaction with β-catenin, and activating the Hippo signaling pathway, suppressing YAP signaling by its N-terminal cytoplasmic region interaction with MST1 . Research studies have revealed that the tumor suppressor functions identified in Drosophila are conserved in vertebrate FAT1 homologs. For example, studies in human cancer cells showed that loss-of-function mutations in the gene encoding FAT1 promoted a hybrid epithelial-to-mesenchymal transition, which further enabled the development of cancer drug resistance. Notably, studies have also revealed an oncogenic function for FAT1 in some contexts.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB

Alternate Names: CDHF7, FAT, Protocadherin Fat 1, Cadherin family member 7, Cadherin-related tumor suppressor homolog, Protein fat homolog

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human FAT1. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of FAT1 protein.

Molecular Weight: ~ 506 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q14517

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only