Product Description

FBP3 (R241) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3297-50, BS3297-100

Sizes: 50µl, 100µl

Swiss-Prot: Q96I24

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Activation of FUSE, the far-upstream element, is required for the proper expression of the mammalian gene c-Myc in undifferentiated cells. The binding of FBP (FUSE-binding protein) to FUSE is necessary for c-Myc expression, indicating that FBP functions as a growth-dependent regulator of c-Myc expression. Isolated from proliferating HL60 cells, FBP, FBP2, and FBP3 comprise a family of single-stranded DNA-binding proteins that specifically bind to FUSE elements. The FBP transcription factors share a conserved central DNA-binding domain and show significant homology in their carboxylterminal activation domains. Expression of FBP is detected in undifferentiated cells and is substantially decreased following cellular differentiation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: FBP3 (R241) polyclonal antibody detects endogenous levels of FBP3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FBP3 (R241) pAb at 1:500 dilution
Lane 1: CT26 whole cell lysate (40ug)
Lane 2: PC12 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: SGC7901 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of FBP3 (R241) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 60 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Far upstream element-binding protein 3; FUSE-binding protein 3; FUBP3; FBP3

Immunogen: Synthetic peptide, corresponding to amino acids 210-254 of Human FBP3.

Conjugate: Unconjugated

Modification: Unmodified