Product Description

Fbx32 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90497-50, BS90497-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q969P5(Human) Q9CPU7(Mouse) Q91Z62(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200

Background: Muscle atrophy F-box (MAFbx) is an E3 ubiquitin ligase that initiates ATP-dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. MAFbx transcript is abundant in cardiac and skeletal muscle undergoing atrophy. MAFbx -/- mice are resistant to muscle atrophy. MAFbx is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. It interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. MAFbx is specifically expressed in cardiac and skeletal muscle.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Fbx32 polyclonal antibody detects endogenous levels of Fbx32 protein.

Bioworld Molecular Weight: 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1,000 dilution., ICC staining Fbx32 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MAFbx, FBXO32, 4833442G10Rik, AI430017, Atrogin 1, Atrogin-1, ATROGIN1, Atrophy gene 1, F box only protein 32, F-box only protein 32, F-box protein 32, FBX32, fbxo25, FBXO32, FLJ32424, MAFbx, MGC108443, MGC137646, MGC33610, Muscle atrophy F box, Muscle atrophy F box protein, Muscle atrophy F-box protein,

Immunogen: Recombinant protein corresponding to human Fbx32 1-200aa.

Conjugate: Unconjugated

Modification: Unmodified