Product Description

FBXL18 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9962M-50, BS9962M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96ME1

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC FC

All Applications: WB: 1:500
IHC: 1:50-1:200
FC: 1:50-1:100

Background: FBL18 (F-box and leucine-rich repeat protein 18), also known as FBXL18, is an 805 amino acid protein that contains an F-box near its N-terminus, followed by several leucine-rich repeats and a transmembrane domain at the C-terminus. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune response, signaling cascades and developmental processes) through the targeting of proteins, such as cyclins and cyclin-dependent kinase inhibitors (CDKNs), for degradation by the proteasome after ubiquitination.

Purification and Purity: Protein A affinity purified.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of FBXL18 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 40, 88 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of FBXL18 on PC-3M cell lysate using anti-FBXL18 antibody at 1:500 dilution., Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-FBXL18 antibody. Counter stained with hematoxylin., Flow cytometric analysis of A549 cells with FBXL18 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Alternative Name: F-box/LRR-repeat protein 18; F-box and leucine-rich repeat protein 18; FBXL18; FBL18

Immunogen: Synthetic peptide corresponding to human FBXL18 aa 750 to C terminus.

Conjugate: Unconjugated

Modification: Unmodified