Product Description

FBXW7 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90499-50, BS90499-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q969H0(Human) Q8VBV4(Mouse) D3Z902(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: FBXW7 polyclonal antibody detects endogenous levels of FBXW7 protein.

Bioworld Molecular Weight: 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of FBXW7 on different lysates using anti-FBXW7 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse spleen tissue
Lane 2: Rat lung tissue
Lane 3: HepG2, ICC staining FBXW7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: hAgo, FBXW7, FBW7, AGO, Archipelago, Archipelago homolog, Archipelago, Drosophila, homolog of, CDC4, CDC4, S.cerevisiae, homolog of, DKFZp686F23254, F box and WD 40 domain protein 7 (archipelago homolog, Drosophila), F box and WD 40 domain protein 7, F box and WD repeat domain containing 7, F box protein FBW7, F box protein FBX30, F box protein SEL 10, F box protein SEL10, F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, F-box/WD repeat-containing protein 7, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, FBXW7, FLJ16457, hAgo, hCdc4, Homolog of C elegans sel 10, Homolog of C.elegans sel10, SEL 10, SEL-10, SEL10,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified