FDPS Monoclonal Antibody-MB66885

FDPS Monoclonal Antibody

Catalogue Numbers: MB66885-50, MB66885-100

Sizes: 50µl, 100µl

Product: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P14324

Host: Mouse

Reactivity: Human, Mouse

Applications: WB

All Applications: WB (1/1000 - 1/2000)

Background: The chromatin immunoprecipitation assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell . This assay can be used to either identify multiple proteins associated with a specific region of the genome or to identify the many regions of the genome bound by a particular protein . ChIP can be used to determine the specific order of recruitment of various proteins to a gene promoter or to "measure" the relative amount of a particular histone modification across an entire gene locus . In addition to histone proteins, the ChIP assay can be used to analyze binding of transcription factors and co-factors, DNA replication factors, and DNA repair proteins. When performing the ChIP assay, cells are first fixed with formaldehyde, a reversible protein-DNA cross-linking agent that "preserves" the protein-DNA interactions occurring in the cell . Cells are lysed and chromatin is harvested and fragmented using either sonication or enzymatic digestion. Fragmented chromatin is then immunoprecipitated with antibodies specific to a particular protein or histone modification. Any DNA sequences that are associated with the protein or histone modification of interest will co-precipitate as part of the cross-linked chromatin complex and the relative amount of that DNA sequence will be enriched by the immunoselection process. After immunoprecipitation, the protein-DNA cross-links are reversed and the DNA is purified. Standard PCR or quantitative real-time PCR are often used to measure the amount of enrichment of a particular DNA sequence by a protein-specific immunoprecipitation . Alternatively, the ChIP assay can be combined with genomic tiling micro-array techniques, high throughput sequencing , or cloning strategies, all of which allow for genome-wide analysis of protein-DNA interactions and histone modifications. SimpleChIP® primers have been optimized for amplification of ChIP-isolated DNA using real-time quantitative PCR and provide important positive and negative controls that can be used to confirm a successful ChIP experiment.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of FDPS protein.

Extra Notes: Western blot analysis of FDPS expression in Hela (A), HepG2 (B), U251 MG (C), mouse liver (D) whole cell lysates.

Bioworld Molecular Weight: ~ 40, 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: FPS; KIAA1293; Farnesyl pyrophosphate synthase; FPP synthase; FPS; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Geranyltranstransferase

Immunogen: Recombinant fusion protein of human FDPS. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified