FEN-1 Polyclonal Antibody Sizes: 50μL, 100μL Catalogue Numbers: RA24915-50, RA24915-100 Lead times: 1-2 weeks, if manufacturer has product in stock Manufacturer/Ship Location: China Background: flap structure-specific endonuclease 1 (FEN1) Homo sapiens The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions Reactivity: Human, Mouse, Rat Host: Rabbit Applications: WB, IHC-p, IF/ICC, ELISA Alternate Names: FEN1, RAD2, Flap endonuclease 1, FEN-1, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1 Clonality: Polyclonal Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. Molecular Weight: 42 Protein Molecular Weight (kDa): 42 Storage: -20°C/1 year Human Gene ID: 2237 Human SwissProt Number: P39748 Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Research Use Only