FEN-1 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A1175-20, A1175-100
Citations, Manuals and MSDS Available upon request.
Background: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, IP, ELISA
Cross-reactivity: Human, Mouse
Protein Weight: 43kDa
Observed Molecular Weight: 48kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 2237
SWISS: P39748
Alternate Names: MF1; RAD2; FEN-1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IHC-P, 1:50 - 1:200 IF/ICC, 1:10 - 1:100 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, DNA Damage Repair.
NCBI Alias: FEN1
Research Use Only