FEN1 (7H8) Monoclonal Antibody-MB0179

FEN1 (7H8) Monoclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: MB0179-50, MB0179-100

Swiss-Prot: P39748

Host: Mouse

FEN1 (7H8) Monoclonal Antibody-MB0179

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, ICC

All Applications: WB: 1:1000
ICC: 1:1000~500

Background: DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. FEN-1 (for flap endonuclease) is an endonuclease that specifically cleaves the 5' flap structure of DNA in the process of DNA repair. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: This antibody detects endogenous levels of FEN1 and does not cross-react with related proteins.

Bioworld Molecular Weight: Predicted band size:42KDa
Observed band size:42KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blotDetection of FEN-1 in Hela,Jurkat,3T3,COS7,PC-12,C6,Raw264.7,Raji and CHO-K1 cellLysates using FEN-1 mouse mAb (1:1000 diluted) ., Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400) .

Alternative Name: Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1; FEN1; RAD2

Immunogen: Purified recombinant human FEN-1 protein fragments expressed in E.coli.

Modification: Unmodified

Conjugate: Unconjugated