Product Description

FEN1 (A119) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1989-50, BS1989-100

Sizes: 50µl, 100µl

Swiss-Prot: P39748

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. FEN-1 (for flap endonuclease) is an endonuclease that specifically cleaves the 5' flap structure of DNA in the process of DNA repair. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: FEN1 (A119) polyclonal antibody detects endogenous levels of FEN1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FEN1 (A119) pAb at 1:500 dilution
Lane 1: SP2/0 whole cell lysate (20ug)
Lane 2: C6 whole cell lysate (40ug)
Lane 3: U-87MG whole cell lysate (40ug)
Lane 4: Hela whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of FEN1 (A119) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 42 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1; FEN1; RAD2

Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human FEN1.

Conjugate: Unconjugated

Modification: Unmodified