Product Description

FEN1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-60232-60, E-AB-60232-120, E-AB-60232-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: FEN1

Target Synonym: FEN1; FEN-1; MF1; RAD2

Research Areas: Cancer, Epigenetics and Nuclear Signaling

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse

Application: WB, IHC, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P39748

Background: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human FEN1 (NP_004102.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:10-1:100

Calculated MW: 35kDa/42kDa

Observed MW: 48kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only